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Book
Gene transfer and expression : a laboratory manual
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ISBN: 1349118915 Year: 1990 Publisher: New York : Stockton Press,

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A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.

The major transitions in evolution
Authors: ---
ISBN: 0191919233 0191586005 9780191586002 019850294X 9780198502944 9780191919237 Year: 1997 Publisher: Oxford New York Oxford University Press

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During evolution, there have been several major changes in the way that genetic information is organised and transmitted from one generation to the next. These transitions include the origin of life itself, the first eukaryotic cells, reproduction by sexual means, the appearance of multicellular plants and animals, the emergence of cooperation and of animal societies, and the unique language ability of humans. In discussing such a wide range of topics in one volume, the authors are able to highlight the similarities between different transitions - for example, between the union of replicating molecules to form chromosomes and of cells to form multicellular organisms.


Book
Sperm-mediated gene transfer
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ISBN: 1608052370 9781608052370 9781608054329 Year: 2012 Publisher: [Oak Park, Ill.] Bentham eBooks

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Sperm-mediated gene transfer (SMGT) represents a novel set of technologies for animal (or in the future, human) genetic modification using the sperm as a vector, as opposed to more traditional established routes such as fertilized eggs or embryonic stem cells. This e-book brings together theoretical and empirical reviews from experts in SMGT, providing comprehensive coverage of the major trends, developments and controversies in this novel field.


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Genetic transformation
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Year: 2011 Publisher: IntechOpen

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Genetic transformation of plants has revolutionized both basic and applied plant research. Plant molecular biology and physiology benefit from this power fool, as well as biotechnology. This book is a review of some of the most significant achievements that plant transformation has brought to the fields of Agrobacterium biology, crop improvement and, flower, fruit and tree amelioration. Also, it examines their impact on molecular farming, phytoremediation and RNAi tools.


Book
Advanced textbook on gene transfer, gene therapy, and genetic pharmacology : principles, delivery, and pharmacological and biomedical applications of nucleotide-based therapies
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ISBN: 1848168306 9781848168305 9781848168282 9781908977281 Year: 2014 Publisher: London : Imperial College Press,

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This unique advanced textbook provides a clear and comprehensive description of the field of gene delivery, gene therapy and genetic pharmacology, with descriptions of the main gene transfer vectors and a set of selected therapeutic applications, along with safety considerations. The use of gene transfer is exponentially growing in the scientific and medical communities for day-to-day cell biology experiments and swift development of revolutionary gene therapy strategies. In this advanced textbook, more than 25 leading scientists, world-renowned in their respective fields, come together to pro

The dynamic bacterial genome
Author:
ISBN: 9780511541544 9780521821575 9780521129619 9780511345234 0511345232 051134452X 9780511344527 0511541546 0521821576 0521821576 1281108561 9781281108562 9786611108564 6611108564 1139130471 9781139130479 0511344899 9780511344893 0511344139 9780511344138 110714566X 0521129613 Year: 2005 Publisher: Cambridge : Cambridge University Press,

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This book provides an in-depth analysis of the mechanisms and biological consequences of genome rearrangements in bacteria. Genome rearrangements are a result of the actions of discrete genetic elements such as conjugative transposons, plasmids, phage, and non-conjugative transposons. Bacteria also contain systems to mediate genetic rearrangements such as the general recombination pathway and specialized endogenous recombination mechanisms. The biological effects of these rearrangements are far-reaching and impact on bacterial virulence, antibiotic resistance and the ability of bacteria to avoid the attentions of the host immune system (e.g. antigenic variation). These rearrangements also provide the raw material on which natural selection can act. Each chapter examines the mechanisms involved in genome rearrangements and the direct biological consequences of these events. This book is written by leading research workers and is an invaluable resource for graduate students and researchers in this field.


Book
Agrobacterium biology and its application to transgenic plant production
Authors: --- --- ---
Year: 2015 Publisher: Frontiers Media SA

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The broad host range pathogenic bacterium Agrobacterium tumefaciens has been widely studied as a model system to understand horizontal gene flow, secretion of effector proteins into host cells, and plant-pathogen interactions. Agrobacterium-mediated plant transformation also is the major method for generating transgenic plants for research and biotechnology purposes. Agrobacterium species have the natural ability to conduct interkingdom genetic transfer from bacteria to eukaryotes, including most plant species, yeast, fungi, and even animal cells. In nature, A. tumefaciens causes crown gall disease resulting from expression in plants of auxin and cytokinin biosynthesis genes encoded by the transferred (T-) DNA. Gene transfer from A. tumefaciens to host cells requires virulence (vir) genes that reside on the resident tumor-inducing (Ti) plasmid. In addition to T-DNA, several Virulence (Vir) effector proteins are also translocated to host cells through a bacterial type IV secretion system. These proteins aid in T-DNA trafficking through the host cell cytoplasm, nuclear targeting, and T-DNA integration. A recently discovered bacterial type VI secretion system may also export bacterial proteins and impact transformation. Genes within native T-DNAs can be replaced by any gene of interest, making Agrobacterium species important tools for plant research and genetic engineering. Agrobacterium-mediated genetic transformation is easy to use, relatively inexpensive, and generally results in a low copy number of transgene insertions when compared to other means of plant transformation, such as particle bombardment. In this research topic, we shall provide updated information on several important areas of Agrobacterium biology and its use for biotechnology purposes.

Horizontal gene transfer
Authors: ---
ISBN: 9780126801262 0126801266 9786611033682 1281033685 0080534120 9780080534121 Year: 2002 Publisher: San Diego : Academic Press,

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The second edition of Horizontal Gene Transfer has been organized to provide a concise and up-to-date coverage of the most important discoveries in this fascinating field. Written by the most prominent gene transfer and genome analytical scientists, this book details experimental evidence for the phenomenon of horizontal gene transfer and discusses further evidence provided by the recent completion of genomic sequences from Archea, Bacteria, and Eucarya members. The relevance of horizontal gene transfer to plant and metazoan taxonomy, GM foods, antibiotic resistance, paleontology, and p


Book
Horizontal gene transfer in the evolution of pathogenesis
Authors: ---
ISBN: 9780511541520 9780521862974 9780511409813 0511409818 0511409273 9780511409271 051154152X 0521862973 1107178215 1281716952 9786611716950 0511407912 0511407173 0511408706 9781107178212 9781281716958 6611716955 9780511407918 9780511407178 9780511408700 Year: 2008 Publisher: Cambridge : Cambridge University Press,

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Horizontal gene transfer is a major driving force in the evolution of many bacterial pathogens. The development of high-throughput sequencing tools and more sophisticated genomic and proteomic techniques in recent years has resulted in a better understanding of this phenomenon. Written by leading experts in the field, this edited volume is aimed at graduate students and researchers and provides an overview of current knowledge relating to the evolution of microbial pathogenicity. This volume provides an overview of the mechanisms and biological consequences of the genome rearrangements resulting from horizontal gene transfer, in both prokaryotes and eukaryotes, as well as overviews of the key mobile genetic elements involved. Subsequent chapters focus on paradigms for the evolution of important bacterial pathogens, including Salmonella enterica, Streptococcus pneumoniae, and Staphylococcus aureus. The influence of socioeconomic parameters in the dissemination of transferable elements, such as antibiotic resistant genes in bacteria, is also discussed.

Molecular genetics, gene transfer, and therapy
Authors: ---
ISBN: 0120392410 9786611046231 128104623X 0080526829 9780120392414 9780080526829 9781281046239 Year: 1997 Volume: 40 Publisher: San Diego, Calif. Academic Press

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Animal science is being transformed by advances in molecular genetics. This book covers this exciting transformation. Both the diagnosis and treatment of disease are discussed in seven chapters by a team of leading international authorities. From oncogenesis and hemophilia to retroviral virulence and genome mapping, this volume yields an understanding of fundamental mechanisms of action and insightful means of diagnosis and treatment. Practicing veterinarians and researchers in animal science will find this book useful.

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